pci egfp glun1 Search Results


pci egfp nr1 wt  (Addgene inc)
93
Addgene inc pci egfp nr1 wt
A. Currents recorded from ND7/23 cells expressing <t>GluN1/GluN2A</t> receptors, at –60 mV, –30 mV and +30 mV, respectively, upon application of NMDA (100 µM, black traces) or NMDA + NASPM (8.7 μM, 26.1 μM, 87 μM, color gradient traces) in a Mg 2+ -free condition. B. Normalized GluN1/GluN2A receptor peak currents, categorized by membrane potential and concentration, with upper and lower quartiles, mean and standard deviation. C. Estimated concentration dependencies of GluN1/GluN2A block by NASPM. Average normalized GluN1/GluN2A peak currents were fitted with a rectangular hyperbolic function, assuming direct occlusion of the ion channel pore by a single NASPM molecule. The IC 50 values from the fits were 3.29 ± 0.54 µM, 23.6 ± 1.1 µM, and 56.1 ± 7.4 µM at –60 mV, –30 mV, and +30 mV.
Pci Egfp Nr1 Wt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pci-egfp-glun1 wild type (wt)  (Addgene inc)
90
Addgene inc pci-egfp-glun1 wild type (wt)
A. Currents recorded from ND7/23 cells expressing <t>GluN1/GluN2A</t> receptors, at –60 mV, –30 mV and +30 mV, respectively, upon application of NMDA (100 µM, black traces) or NMDA + NASPM (8.7 μM, 26.1 μM, 87 μM, color gradient traces) in a Mg 2+ -free condition. B. Normalized GluN1/GluN2A receptor peak currents, categorized by membrane potential and concentration, with upper and lower quartiles, mean and standard deviation. C. Estimated concentration dependencies of GluN1/GluN2A block by NASPM. Average normalized GluN1/GluN2A peak currents were fitted with a rectangular hyperbolic function, assuming direct occlusion of the ion channel pore by a single NASPM molecule. The IC 50 values from the fits were 3.29 ± 0.54 µM, 23.6 ± 1.1 µM, and 56.1 ± 7.4 µM at –60 mV, –30 mV, and +30 mV.
Pci Egfp Glun1 Wild Type (Wt), supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pci-egfp-glun1 wild type (wt)/product/Addgene inc
Average 90 stars, based on 1 article reviews
pci-egfp-glun1 wild type (wt) - by Bioz Stars, 2026-02
90/100 stars
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glun1 cdnas  (Addgene inc)
93
Addgene inc glun1 cdnas
A. Currents recorded from ND7/23 cells expressing <t>GluN1/GluN2A</t> receptors, at –60 mV, –30 mV and +30 mV, respectively, upon application of NMDA (100 µM, black traces) or NMDA + NASPM (8.7 μM, 26.1 μM, 87 μM, color gradient traces) in a Mg 2+ -free condition. B. Normalized GluN1/GluN2A receptor peak currents, categorized by membrane potential and concentration, with upper and lower quartiles, mean and standard deviation. C. Estimated concentration dependencies of GluN1/GluN2A block by NASPM. Average normalized GluN1/GluN2A peak currents were fitted with a rectangular hyperbolic function, assuming direct occlusion of the ion channel pore by a single NASPM molecule. The IC 50 values from the fits were 3.29 ± 0.54 µM, 23.6 ± 1.1 µM, and 56.1 ± 7.4 µM at –60 mV, –30 mV, and +30 mV.
Glun1 Cdnas, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/glun1 cdnas/product/Addgene inc
Average 93 stars, based on 1 article reviews
glun1 cdnas - by Bioz Stars, 2026-02
93/100 stars
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Image Search Results


A. Currents recorded from ND7/23 cells expressing GluN1/GluN2A receptors, at –60 mV, –30 mV and +30 mV, respectively, upon application of NMDA (100 µM, black traces) or NMDA + NASPM (8.7 μM, 26.1 μM, 87 μM, color gradient traces) in a Mg 2+ -free condition. B. Normalized GluN1/GluN2A receptor peak currents, categorized by membrane potential and concentration, with upper and lower quartiles, mean and standard deviation. C. Estimated concentration dependencies of GluN1/GluN2A block by NASPM. Average normalized GluN1/GluN2A peak currents were fitted with a rectangular hyperbolic function, assuming direct occlusion of the ion channel pore by a single NASPM molecule. The IC 50 values from the fits were 3.29 ± 0.54 µM, 23.6 ± 1.1 µM, and 56.1 ± 7.4 µM at –60 mV, –30 mV, and +30 mV.

Journal: bioRxiv

Article Title: The polyamine naphthyl-acetyl spermine trihydrochloride (NASPM) lacks specificity for Ca 2+ -permeable AMPA receptors and suppresses seizure like activity in human brain tissue by inhibition of NMDA receptors

doi: 10.1101/2025.05.14.653650

Figure Lengend Snippet: A. Currents recorded from ND7/23 cells expressing GluN1/GluN2A receptors, at –60 mV, –30 mV and +30 mV, respectively, upon application of NMDA (100 µM, black traces) or NMDA + NASPM (8.7 μM, 26.1 μM, 87 μM, color gradient traces) in a Mg 2+ -free condition. B. Normalized GluN1/GluN2A receptor peak currents, categorized by membrane potential and concentration, with upper and lower quartiles, mean and standard deviation. C. Estimated concentration dependencies of GluN1/GluN2A block by NASPM. Average normalized GluN1/GluN2A peak currents were fitted with a rectangular hyperbolic function, assuming direct occlusion of the ion channel pore by a single NASPM molecule. The IC 50 values from the fits were 3.29 ± 0.54 µM, 23.6 ± 1.1 µM, and 56.1 ± 7.4 µM at –60 mV, –30 mV, and +30 mV.

Article Snippet: They were passaged twice a week and seeded onto glass coverslips in 60mm Petri dishes two days prior to experiments. pCI-EGFP-NR2a wt (Addgene plasmid #45445, http://n2t.net/addgene:45445 ; RRID:Addgene_45445) and pCI-EGFP-NR1 wt (Addgene plasmid #45446, http://n2t.net/addgene:45446 ; RRID:Addgene_45446) were gifts from Andres Barria & Robert Malinow (Barria et Malinow, 2002).

Techniques: Expressing, Membrane, Concentration Assay, Standard Deviation, Blocking Assay